Francesca D'Alessio
Career Summary
Cell/Tissue Culture Facility Manager 2018 – present
CEINGE – Biotecnologie Avanzate s.c.a r.l
Cell/Tissue Culture Facility Supervisor and Coordinator 2013 – 2018
CEINGE – Biotecnologie Avanzate s.c.a r.l.,
Tutor: Professor Luigi Del Vecchio
Cell/Tissue Culture and Flow Cytometry Reseacher 2006 - 2012
Cell Culture Facility and Clinical and Experimental Flow Cytometry Unit
CEINGE – Biotecnologie Avanzate s.c.a r.l.,
Tutors: Professor Rosa Di Noto and Professor Luigi Del Vecchio
Education
Postgraduate School in Clinical Biochemistry 2007-2012
University of Naples “Federico II” , Faculty of Medicine and Surgery
- Graduated Summa Cum Laude
MSc in Medical Biotechnology 2004-2007
University of Naples “Federico II”, Faculty of Biotechnology
- Graduated Summa Cum Laude
BSc in Biotechnology 2001-2004
University of Naples “Federico II”, Faculty of Biotechnology
Documented experience in Cell/Tissue Culture Facility includes:
- Acquisition, culture and cryopreservation of human and animal growing non-adherent and adherent cell lines
- Mycoplasma detection in cell lines and reagent
- Mycoplasma eradication in cell lines
- Establishment of primary cell culture starting from fresh tissue biopsies
- Flow cytometric characterization and analysis of mature and stem cell populations
- Pharmacologic and proliferation activity analysis on cell cultures by vitality assays
Iaffaldano L, Nardelli C, D'Alessio F, D'Argenio V, Nunziato M, Mauriello L, Procaccini C, Maruotti GM, Martinelli P, Matarese G, Pastore L, Del Vecchio L, Labruna G, Sacchetti L. “Altered Bioenergetic Profile in Umbilical Cord and Amniotic Mesenchymal Stem Cells from Newborns of Obese Women”. Stem Cells Dev. 2018 Feb 1; 27(3):199-206.
Briguori C, Quintavalle C, D'Alessio F, Donahue M, Roscigno G, De Micco F, Focaccio A, Visconti G, Del Vecchio L, Madonna R, De Caterina R, Condorelli G. “Impact of statin therapy intensity on endothelial progenitor cells after percutaneous coronary intervention in diabetic patients. The REMEDY-EPC late study”. Int J Cardiol. 2017 Oct 1; 244:112-118.
Visconti R, Della Monica R, Palazzo L, D'Alessio F, Raia M, Improta S, Villa MR, Del Vecchio L, Grieco D. “The Fcp1-Wee1-Cdk1 axis affects spindle assembly checkpoint robustness and sensitivity to antimicrotubule cancer drugs”. Cell Death Differ. 2015 Sep; 22(9): 1551-60.
Mariotti E, D'Alessio F, Mirabelli P, Di Noto R, Fortunato G, Del Vecchio L. “Mollicutes contamination: A new strategy for an effective rescue of cancer cell lines”. Biologicals 2012 Jan; 40: 88-91.
D’Alessio F, Mirabelli P, Gorrese M,Scalia G, Gemei M, Mariotti E, Fortunato G, Di Noto R, Paladini D, Del Vecchio L. “Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples”. Cytometry part A, 2011 Jan; 79(1): 14-24.