Francesca D'Alessio
Cell Cultures

Francesca D'Alessio

E-mail
dalessiof@ceinge.unina.it
BIOSKETCH

Career Summary

Cell/Tissue Culture Facility Manager                                                        2018 – present

CEINGE – Biotecnologie Avanzate s.c.a r.l

Cell/Tissue Culture Facility Supervisor and Coordinator                         2013 – 2018

CEINGE – Biotecnologie Avanzate s.c.a r.l.,

Tutor: Professor Luigi Del Vecchio

 

Cell/Tissue Culture and Flow Cytometry Reseacher                               2006 - 2012

Cell Culture Facility and Clinical and Experimental Flow Cytometry Unit

CEINGE – Biotecnologie Avanzate s.c.a r.l.,

Tutors: Professor Rosa Di Noto and Professor Luigi Del Vecchio

 

Education

Postgraduate School in Clinical Biochemistry                                                  2007-2012

University of Naples “Federico II” , Faculty of Medicine and Surgery

  • Graduated Summa Cum Laude

 

MSc in Medical Biotechnology                                                                          2004-2007

University of Naples “Federico II”, Faculty of Biotechnology

  • Graduated Summa Cum Laude

 

BSc in Biotechnology                                                                                          2001-2004

University of Naples “Federico II”, Faculty of Biotechnology

 

Documented experience in Cell/Tissue Culture Facility includes:

  • Acquisition, culture and cryopreservation of human and animal growing non-adherent and adherent cell lines
  • Mycoplasma detection in cell lines and reagent
  • Mycoplasma eradication in cell lines
  • Establishment of primary cell culture starting from fresh tissue biopsies
  • Flow cytometric characterization and analysis of mature and stem cell populations
  • Pharmacologic and proliferation activity analysis on cell cultures by vitality assays

 

MOST RELEVANT PUBLICATIONS

Iaffaldano L, Nardelli C, D'Alessio F, D'Argenio V, Nunziato M, Mauriello L, Procaccini C, Maruotti GM, Martinelli P, Matarese G, Pastore L, Del Vecchio L, Labruna G, Sacchetti L. “Altered Bioenergetic Profile in Umbilical Cord and Amniotic Mesenchymal Stem Cells from Newborns of Obese Women”. Stem Cells Dev. 2018 Feb 1; 27(3):199-206.

Briguori C, Quintavalle C, D'Alessio F, Donahue M, Roscigno G, De Micco F, Focaccio A, Visconti G, Del Vecchio L, Madonna R, De Caterina R, Condorelli G. “Impact of statin therapy intensity on endothelial progenitor cells after percutaneous coronary intervention in diabetic patients. The REMEDY-EPC late study”. Int J Cardiol. 2017 Oct 1; 244:112-118.

Visconti R, Della Monica R, Palazzo L, D'Alessio F, Raia M, Improta S, Villa MR, Del Vecchio L, Grieco D. “The Fcp1-Wee1-Cdk1 axis affects spindle assembly checkpoint robustness and sensitivity to antimicrotubule cancer drugs”. Cell Death Differ. 2015 Sep; 22(9): 1551-60.

Mariotti E, D'Alessio F, Mirabelli P, Di Noto R, Fortunato G, Del Vecchio L. “Mollicutes contamination: A new strategy for an effective rescue of cancer cell lines”. Biologicals 2012 Jan; 40: 88-91.

D’Alessio F, Mirabelli P, Gorrese M,Scalia G, Gemei M, Mariotti E, Fortunato G, Di Noto R, Paladini D, Del Vecchio L. “Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples”. Cytometry part A, 2011 Jan; 79(1): 14-24.

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