Stefano Guido
Bioengineering at the micro-scale

Stefano Guido

E-mail
steguido@unina.it
Biosketch

Stefano Guido received a Laurea cum laude in Chemical Engineering in 1989 at the University of Napoli “Federico II”, a Master of Science in Chemical Engineering in 1992 at the University of Minnesota, and a PhD in Chemical Engineering at the University of Napoli “Federico II” on biased cell migration in oriented collagen gels. He is currently Professor at the Department of Chemical, Materials and Production Engineering and PI at the CEINGE Advanced Biotechnologies Center. He has been visiting professor at several universities, including the University of California at Irvine, the University of Edinburgh, the University of Cambridge and the University of Oxford. He is currently involved in two Topical Teams of the European Space Agency on biomimetic and cellular systems and on biofilms. His main research interests are in cell motility, hemorheology, ektacytometry, time-lapse microscopy and microfluidics techniques applied to biological systems.

Research

The focus of our research is to characterize single and collective cell behavior and transport phenomena in complex biological fluids, such as blood, collagen gels and mucus, by advanced microscopy techniques (confocal, epifluorescence, polarized, time-lapse, high-speed), coupled to image analysis and biorheology. In particular, we use Lab-on-chip devices fabricated by soft-lithography for microfluidics experiments. The results of this work are relevant in a number of applications, from design of diagnostic and therapeutic devices to interactions between biological systems and industrial products.

Research lines
  1. Microfluidics

  2. Rheology of biological fluids

  3. Cell migration

  4. Effect of flow on cell behavior and bacterial biofilms
Research Group
  • Sergio Caserta, Associate Professor
  • Giovanna Tomaiuolo, RTDB
  • Valentina Preziosi, Postdoc
  • Andrea Iginio Cirillo, PhD student
  • Fiammetta Fellico, PhD student
  • Rosalia Ferraro, PhD student
  • Raffaele Graziano, PhD student
Most relevant publications
  1. G. Tomaiuolo, M. Barra, V. Preziosi, A. Cassinese, B. Rotoli, S. Guido, “Microfluidics analysis of red blood cell membrane viscoelasticity”, Lab Chip, 11, 449-454 (2011)
  2. R. D'Apolito, G. Tomaiuolo, F. Taraballi, S. Minardi, D. Kirui, X. Liu, A. Cevenini, R. Palomba, M. Ferrari, F. Salvatore, E. Tasciotti, S. Guido, Red blood cells affect the margination of microparticles in synthetic microcapillaries and intravital microcirculation as a function of their size and shape, J. Control. Release, 217, 263-72 (2015)
  3. F. Ascione, S. Caserta and S. Guido, “The Wound Healing assay revisited: a transport phenomena approach”, Chem. Eng. Sci., 160, 200-209 (2017)
  4. A. Perazzo, J. K. Nunes, S. Guido and H. A. Stone, Flow-induced gelation of microfiber suspensions, PNAS, 114 (41), E8557-E8564 (2017)
  5. V. Introini, A. Carciati, G. Tomaiuolo, P. Cicuta, S. Guido, “Endothelial glycocalyx regulates cytoadherence in Plasmodium falciparum malaria”, J. R. Soc. Interface, 15 (149), 20180773 (2018)